SUSY Constraints, Relic Density, and Very Early Universe

The sensitivity of the lightest supersymmetric particle relic density calculation to different cosmological scenarios is discussed. In particular, we investigate the effects of modifications of the expansion rate and of the entropy content in the Early Universe. These effects, even with no observational consequences, can still drastically modify the relic density constraints on the SUSY parameter space. We suggest general parametrizations to evaluate such effects, and derive also constraints from Big-Bang nucleosynthesis. We show that using the relic density in the context of supersymmetric constraints requires a clear statement of the underlying cosmological model assumptions to avoid misinterpretations. On the other hand, we note that combining the relic density calculation with the eventual future discoveries at the LHC will hopefully shed light on the Very Early Universe properties.Comment: 11 pages, 5 figures. v2: new figures adde

Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells

Upregulation of specific transcription factors is a generally accepted mechanism to explain the commitment of hematopoietic stem cells along precise maturation lineages. Based on this premise, transduction of primary hematopoietic stem/progenitor cells with viral vectors containing the investigated transcription factors appears as a suitable experimental model to identify such regulators. Although MafB transcription factor is believed to play a role in the regulation of monocytic commitment, no demonstration is, to date, available supporting this function in normal human hematopoiesis. To address this issue, we retrovirally transduced cord blood CD34+ hematopoietic progenitors with a MafB cDNA. Immunophenotypic and morphological analysis of transduced cells demonstrated the induction of a remarkable monomacrophage differentiation. Microarray analysis confirmed these findings and disclosed the upregulation of macrophage-related transcription factors belonging to the AP-1, MAF, PPAR and MiT families. Altogether our data allow to conclude that MafB is a key regulator of human monocytopoiesis